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Vignettes

We offer a series of tutorials to help users master the cartloader toolkit across a range of scenarios — from simple getting-started examples to processing full-scale ST datasets.

For a more detailed explanation of each step, refer to our References Page.

Quick Start

If you're new to cartloader, we recommend beginning with the Quick Start tutorial.

This vignette provides a beginner-friendly walkthrough using a small mouse hippocampus dataset to walks through cartloader functions.

  • Quick Start
    Quick Start Tutorial

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Getting Started per Platform

These tutorials show how to run the cartloader pipeline on a variety of ST platforms, using small, representative datasets.

Each tutorial begins with data in the platform’s native format.

For consistency, we curated hippocampus from the mouse brain for each platform, when possible. If hippocampal data is unavailable for a given platform, we selected another brain subregion to illustrate the process.

Sequencing-based Platforms

  • example1
    SeqScope Starter Guide

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  • example2
    10X Visium HD Starter Guide

    Read

  • example3
    StereoSeq Starter Guide

    Read

  • example3
    Pixel-seq Starter Guide

    Read

Imaging-based Platforms

  • example3
    10X Xenium Starter Guide

    Read

  • example3
    Vizgen MERSCOPE Starter Guide

    Read

  • example3
    CosMX SMI Starter Guide

    Read

Real-World Use Cases

These examples demonstrate how to process walk through real-world whole-sample ST datasets. Each example uses a whole mouse brain sample for consistency.

Multi-Sample Batch Analysis

Warning

This function is still under development

This upcoming section will showcase how to process multiple samples in a single batch. It will include tutorials on cross-sample analysis.