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Vignettes

We offer a series of tutorials to help users master the cartloader toolkit across a range of scenarios — from simple getting-started examples to processing full-scale ST datasets.

For a more detailed explanation of each step, refer to our References Page.

Quick Start

If you're new to cartloader, we recommend beginning with the Quick Start tutorial.

This vignette provides a beginner-friendly walkthrough using a small mouse hippocampus dataset to walks through cartloader functions.

  • Quick Start
    Quick Start Tutorial

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Getting Started per Platform

These tutorials show how to run the cartloader pipeline on a variety of ST platforms, using small, representative datasets.

For consistency, we curated hippocampus from the mouse brain for each platform, when possible. If hippocampal data is unavailable for a given platform, we selected another brain subregion to illustrate the process.

Warning

Each tutorial begins with data in the platform’s native format.

Sequencing-based Platforms

  • example1
    SeqScope Starter Guide

    Read

  • example2
    10X Visium HD Starter Guide

    Read

  • example3
    StereoSeq Starter Guide

    Read

  • example3
    Pixel-seq Starter Guide

    Read

Imaging-based Platforms

  • example3
    10X Xenium Starter Guide

    Read

  • example3
    Vizgen MERSCOPE Starter Guide

    Read

  • example3
    CosMX SMI Starter Guide

    Read

Real-World Use Cases

These examples demonstrate how to process walk through real-world whole-sample ST datasets. Each example uses a whole mouse brain sample for consistency.

Warning

Tutorials will be updated.

Multi-Sample Batch Analysis

Warning

This function is still under development

This upcoming section will showcase how to process multiple samples in a single batch. It will include tutorials on cross-sample analysis.