Welcome to the NovaScope Exemplary Downstream Analysis (NEDA) Documentation

Introduction

This document serves as a guide for exemplary downstream analysis of spatial transcriptomics data generated from NovaScope. The main functionalities include:

1) Pixel-level Analysis: This feature enables the identification of spatial factors at a pixel-level resolution using a hexagon-indexed spatial digital gene expression (SGE) matrix.

2) Cell Segmentation-based Analysis: This feature facilitates the aggregation of SGE matrix at the cellular level based on histology files and supports the analysis of cell type clusters using the cell-indexed SGE.

References

For additional information, please refer to the following links:

• NovaScope Protocol Preprint
• NovaScope
• FICTURE