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Typical Background Layer Naming Scheme

  • Spatial gene expression density map: Spatial gene expression density map in black-white color scheme.
  • H&E histology staining: A brightfield histology image stained with hematoxylin (nuclei, purple/blue) and eosin (cytoplasm/extracellular matrix, pink) to reveal tissue structure and morphology.
  • DAPI nuclear staining: A fluorescence microscopy image where the DNA-binding dye DAPI labels cell nuclei, making them appear bright blue and allowing you to assess nuclear distribution, density, and morphology.
  • DAPI nuclear staining (maximum intensity projection): A 2D projection of the DAPI fluorescence across a z-stack, where each pixel shows the maximum nuclear signal to enhance visibility of nuclei throughout the volume.
  • Interior RNA staining: A staining layer highlighting intracellular RNA signal within cells, useful for evaluating overall RNA content and cytoplasmic signal distribution.
  • 18S rRNA staining: A staining layer marking 18S ribosomal RNA, often used as a strong, widespread RNA marker to assess RNA abundance and tissue/cell integrity.
  • Interior protein staining: A staining layer highlighting intracellular protein signal, useful for visualizing protein abundance and subcellular distribution within cells.
  • Cell boundary staining: A staining layer that outlines cell membranes/boundaries to support cell segmentation and interpretation of cell shape and organization.