How to choose ROI loading options

Concept

• ROI Loading Data Options

Use Current-Level Loading when:

• The ROI covers a large tissue area.
• You are still exploring and may change the ROI boundaries.
• You want to avoid browser memory warnings or crashes.

Use Full ROI Loading when:

• The ROI is small and focused.
• You need the complete raw pixel/transcript data for export.
• You are running a comparison or differential expression analysis that requires full counts.

Tip

When in doubt, start with Current-Level Loading. - You can change the loading option from current-level loading to full roi loading later. - Also, you can reload an existing ROI by re-creating it or by using the Load Existing ROI guide to re-import it with a different option.